RFC5 monoclonal antibody (M01), clone 1B1
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More Files
- More Functions
Western Blot (Cell lysate)
RFC5 monoclonal antibody (M01), clone 1B1. Western Blot analysis of RFC5 expression in Jurkat.
Immunofluorescence
Immunofluorescence of monoclonal antibody to RFC5 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (63.14 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant RFC5.
Immunogen
RFC5 (AAH13961, 1 a.a. ~ 340 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINEDRLPHLLLYGPPGTGKTSTILACAKQLYKDKEFGSMVLELNASDDRGIDIIRGPILSFASTRTIFKKGFKLVILDEADAMTQDAQNALRRVIEKFTENTRFCLICNYLSKIIPALQSRCTRFRFGPLTPELMAPRLEHVVEEEKVDISEDGMKALVTLSSGDMRRALNILQSTNMAFGKVTEETVYTCTGHPLKSDIANILDWMLNQDFTTAYRNITELKTLKGLALHDILTEIHLFVHRVDFPSSVRIHLLTKMADIEYRLSVGTNEKIQLSSLIAAFQVTRDLIVAEA
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (63.14 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RFC5 monoclonal antibody (M01), clone 1B1. Western Blot analysis of RFC5 expression in Jurkat.Western Blot (Recombinant protein)
ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RFC5 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — RFC5
Entrez GeneID
5985GeneBank Accession#
BC013961Protein Accession#
AAH13961Gene Name
RFC5
Gene Alias
MGC1155, RFC36
Gene Description
replication factor C (activator 1) 5, 36.5kDa
Omim ID
600407Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
A1 36 kDa subunit|RFC, 36.5 kD subunit|activator 1 36 kDa subunit|replication factor C (activator 1) 5 (36.5kD)|replication factor C 5
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Interactome
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Pathway
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Disease
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