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RFC4 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005984-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RFC4 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 39.7
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RFC4 antibody (H00005984-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005984-T02
    RFC4 transfected lysate.
    Western Blot
    QC Testing of H00005984-T02
    Lane 1: RFC4 transfected lysate ( 39.70 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5984
  • Gene Name:
  • RFC4
  • Gene Alias:
  • A1,MGC27291,RFC37
  • Gene Description:
  • replication factor C (activator 1) 4, 37kDa
  • Gene Summary:
  • The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
  • Other Designations:
  • A1 37 kDa subunit,RFC 37 kDa subunit,activator 1 37 kDa subunit,replication factor C (activator 1) 4 (37kD),replication factor C 4
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