RFC4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RFC4 transfected lysate
Western Blot
Lane 1: RFC4 transfected lysate ( 39.7 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFC4 full-length
Host
Human
Theoretical MW (kDa)
39.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFC4 antibody (H00005984-B01) by Western Blots.
SDS-PAGE Gel
RFC4 transfected lysate
Western Blot
Lane 1: RFC4 transfected lysate ( 39.7 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFC4
Entrez GeneID
5984GeneBank Accession#
NM_002916Protein Accession#
NP_002907Gene Name
RFC4
Gene Alias
A1, MGC27291, RFC37
Gene Description
replication factor C (activator 1) 4, 37kDa
Omim ID
102577Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
A1 37 kDa subunit|RFC 37 kDa subunit|activator 1 37 kDa subunit|replication factor C (activator 1) 4 (37kD)|replication factor C 4
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Interactome
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Disease
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