RFC4 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of RFC4 transfected lysate using rabbit polyclonal anti-RFC4 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RFC4.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RFC4 transfected lysate using rabbit polyclonal anti-RFC4 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RFC4.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-RFC4 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-RFC4 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — RFC4
Entrez GeneID
5984Gene Name
RFC4
Gene Alias
A1, MGC27291, RFC37
Gene Description
replication factor C (activator 1) 4, 37kDa
Omim ID
102577Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
A1 37 kDa subunit|RFC 37 kDa subunit|activator 1 37 kDa subunit|replication factor C (activator 1) 4 (37kD)|replication factor C 4
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Interactome
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Pathway
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Disease
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