RFC3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RFC3 transfected lysate.
Western Blot
Lane 1: RFC3 transfected lysate ( 40.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFC3 full-length
Host
Human
Theoretical MW (kDa)
40.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFC3 antibody (H00005983-B02P) by Western Blots.
SDS-PAGE Gel
RFC3 transfected lysate.
Western Blot
Lane 1: RFC3 transfected lysate ( 40.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFC3
Entrez GeneID
5983GeneBank Accession#
NM_002915Protein Accession#
NP_002906.1Gene Name
RFC3
Gene Alias
MGC5276, RFC38
Gene Description
replication factor C (activator 1) 3, 38kDa
Omim ID
600405Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
A1 38 kDa subunit|OTTHUMP00000018237|RFC, 38 kD subunit|replication factor C (activator 1) 3 (38kD)|replication factor C 3
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Interactome
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