RFC3 monoclonal antibody (M01), clone 1C6
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RFC3.
Immunogen
RFC3 (AAH00149, 257 a.a. ~ 356 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ETANAIVSQQTPQRLLEVRGRLYELLTHCIPPEIIMKGLLSELLHNCDGQLKGEVAQMAAYYEHRLQLGSKAIYHLEAFVAKFMALYKKFMEDGLEGMMF
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of RFC3 transfected lysate using anti-RFC3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RFC3 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RFC3 is approximately 10ng/ml as a capture antibody.ELISA
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Gene Info — RFC3
Entrez GeneID
5983GeneBank Accession#
BC000149Protein Accession#
AAH00149Gene Name
RFC3
Gene Alias
MGC5276, RFC38
Gene Description
replication factor C (activator 1) 3, 38kDa
Omim ID
600405Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
A1 38 kDa subunit|OTTHUMP00000018237|RFC, 38 kD subunit|replication factor C (activator 1) 3 (38kD)|replication factor C 3
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Interactome
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Disease
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