RFC2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RFC2 transfected lysate.
Western Blot
Lane 1: RFC2 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RFC2 full-length
Host
Human
Theoretical MW (kDa)
35.31
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RFC2 antibody (H00005982-B02) by Western Blots.
SDS-PAGE Gel
RFC2 transfected lysate.
Western Blot
Lane 1: RFC2 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RFC2
Entrez GeneID
5982GeneBank Accession#
NM_002914Protein Accession#
NP_002905Gene Name
RFC2
Gene Alias
A1, MGC3665, RFC40
Gene Description
replication factor C (activator 1) 2, 40kDa
Omim ID
600404Gene Ontology
HyperlinkGene Summary
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
activator 1|replication factor C 2
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Interactome
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