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Last updated: 2017/8/13

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RASA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005921-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RASA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 100.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RASA1 antibody (H00005921-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005921-T01
    RASA1 transfected lysate.
    Western Blot
    QC Testing of H00005921-T01
    Lane 1: RASA1 transfected lysate ( 100.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5921
  • Gene Name:
  • RASA1
  • Gene Alias:
  • CM-AVM,CMAVM,DKFZp434N071,GAP,PKWS,RASA,RASGAP,p120GAP,p120RASGAP
  • Gene Description:
  • RAS p21 protein activator (GTPase activating protein) 1
  • Gene Summary:
  • The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq
  • Other Designations:
  • GTPase activating protein,RAS p21 protein activator 1,triphosphatase-activating protein
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