Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
OTTHUMP00000013740,OTTHUMP00000013741,OTTHUMP00000059089,RAS-related protein RAP1A,Ras-related protein Krev-1,Ras-related protein Rap-1A