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RAG2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005897-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RAG2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 58.08
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RAG2 antibody (H00005897-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005897-T01
    RAG2 transfected lysate.
    Western Blot
    QC Testing of H00005897-T01
    Lane 1: RAG2 transfected lysate ( 58.08 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5897
  • Gene Name:
  • RAG2
  • Gene Alias:
  • RAG-2
  • Gene Description:
  • recombination activating gene 2
  • Gene Summary:
  • This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq
  • Other Designations:
  • V(D)J recombination-activating protein 2
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