RAD51L1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RAD51L1 transfected lysate
Western Blot
Lane 1: RAD51L1 transfected lysate ( 42.2 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAD51L1 full-length
Host
Human
Theoretical MW (kDa)
42.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAD51L1 antibody (H00005890-B01) by Western Blots.
SDS-PAGE Gel
RAD51L1 transfected lysate
Western Blot
Lane 1: RAD51L1 transfected lysate ( 42.2 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAD51L1
Entrez GeneID
5890GeneBank Accession#
NM_133509Protein Accession#
NP_598193Gene Name
RAD51L1
Gene Alias
MGC34245, R51H2, RAD51B, REC2, hREC2
Gene Description
RAD51-like 1 (S. cerevisiae)
Omim ID
602948Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
DNA repair protein RAD51 homolog 2|RAD51 homolog B|RAD51-like 1|RecA-like protein|recombination repair protein
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Interactome
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Pathway
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Disease
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