RAD51C 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RAD51C transfected lysate.
Western Blot
Lane 1: RAD51C transfected lysate ( 42.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAD51C full-length
Host
Human
Theoretical MW (kDa)
42.2
Interspecies Antigen Sequence
Mouse (87); Rat (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAD51C antibody (H00005889-D01P) by Western Blots.
SDS-PAGE Gel
RAD51C transfected lysate.
Western Blot
Lane 1: RAD51C transfected lysate ( 42.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAD51C
Entrez GeneID
5889GeneBank Accession#
NM_058216Protein Accession#
NP_478123.1Gene Name
RAD51C
Gene Alias
MGC104277, RAD51L2
Gene Description
RAD51 homolog C (S. cerevisiae)
Omim ID
602774Gene Ontology
HyperlinkGene Summary
This gene is a member of the RAD51 family of related genes, which encode strand-transfer proteins thought to be involved in recombinational repair of damaged DNA and in meiotic recombination. This gene product interacts with two other DNA repair proteins, encoded by RAD51B and XRCC3, but not with itself. The protein copurifies with XRCC3 protein in a complex, reflecting their endogenous association and suggesting a cooperative role during recombinational repair. This gene is one of four localized to a region of chromosome 17q23 where amplification occurs frequently in breast tumors. Overexpression of the four genes during amplification has been observed and suggests a possible role in tumor progression. Alternative splicing has been observed for this gene and two variants encoding different isoforms have been identified. [provided by RefSeq
Other Designations
DNA repair protein RAD51 homolog 3|RAD51 homolog C|RAD51 homolog C, isoform 1|yeast RAD51 homolog 3
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