RAD51 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human RAD51 protein.
Immunogen
RAD51 (NP_002866.2, 1 a.a. ~ 339 a.a) full-length human protein.
Sequence
MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKELINIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETGSITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGLSGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSARQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRLYLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD
Host
Mouse
Reactivity
Human, Mouse, Rat
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in human colon.Western Blot (Cell lysate)
RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in Raw 264.7.Western Blot (Cell lysate)
RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in Jurkat.Western Blot (Cell lysate)
RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in NIH/3T3.Western Blot (Cell lysate)
RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in PC-12.Western Blot (Transfected lysate)
Western Blot analysis of RAD51 expression in transfected 293T cell line (H00005888-T01) by RAD51 MaxPab polyclonal antibody.
Lane 1: RAD51 transfected lysate(37.29 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of purified MaxPab antibody to RAD51 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml] -
Gene Info — RAD51
Entrez GeneID
5888GeneBank Accession#
NM_002875Protein Accession#
NP_002866.2Gene Name
RAD51
Gene Alias
BRCC5, HRAD51, HsRad51, HsT16930, RAD51A, RECA
Gene Description
RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
BRCA1/BRCA2-containing complex, subunit 5|DNA repair protein RAD51 homolog 1|RAD51 homolog protein|RAD51, S.cerevisiae, homolog of, A|RecA, E. coli, homolog of|RecA-like protein|recombination protein A
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Interactome
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Pathway
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Disease
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Publication Reference
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DNA binding and RAD51 engagement by the BRCA2 C-terminus orchestrate DNA repair and replication fork preservation.
Youngho Kwon, Heike Rösner, Weixing Zhao, Platon Selemenakis, Zhuoling He, Ajinkya S Kawale, Jeffrey N Katz, Cody M Rogers, Francisco E Neal, Aida Badamchi Shabestari, Valdemaras Petrosius, Akhilesh K Singh, Marina Z Joel, Lucy Lu, Stephen P Holloway, Sandeep Burma, Bipasha Mukherjee, Robert Hromas, Alexander Mazin, Claudia Wiese, Claus S Sørensen, Patrick Sung.
Nature Communications 2023 Jan; 14(1):432.
Application:WB, Human, HeLa cells.
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BNIP3-dependent mitophagy safeguards ESC genomic integrity via preventing oxidative stress-induced DNA damage and protecting homologous recombination.
Qian Zhao, Kun Liu, Lin Zhang, Zheng Li, Liang Wang, Jiani Cao, Youqing Xu, Aihua Zheng, Quan Chen, Tongbiao Zhao.
Cell Death & Disease 2022 Nov; 13(11):976.
Application:IF, WB-Ce, WB-Tr, Mouse, Mouse embryonic stem cells.
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Bloom helicase mediates formation of large single-stranded DNA loops during DNA end processing.
Chaoyou Xue, Sameer J Salunkhe, Nozomi Tomimatsu, Ajinkya S Kawale, Youngho Kwon, Sandeep Burma, Patrick Sung, Eric C Greene.
Nature Communications 2022 Apr; 13(1):2248.
Application:IF-Tr, Human, U2OS cells.
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A small-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death.
Duncan E Scott, Nicola J Francis-Newton, May E Marsh, Anthony G Coyne, Gerhard Fischer, Tommaso Moschetti, Andrew R Bayly, Timothy D Sharpe, Kalina T Haas, Lorraine Barber, Chiara R Valenzano, Rajavel Srinivasan, David J Huggins, Miyoung Lee, Amy Emery, Bryn Hardwick, Matthias Ehebauer, Claudio Dagostin, Alessandro Esposito, Luca Pellegrini, Trevor Perrior, Grahame McKenzie, Tom L Blundell, Marko Hyvönen, John Skidmore, Ashok R Venkitaraman, Chris Abell.
Cell Chemical Biology 2021 Jun; 28(6):835.
Application:IF, Human, A549 cells.
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Matrin3 promotes homologous recombinational repair by regulation of RAD51.
Shi L, Sun J, Kinomura A, Fukuto A, Horikoshi Y, Tashiro S.
Journal of Biochemistry 2019 May; mvz041.
Application:IF, WB-Tr, Human, GM0637, U-2 OS cells.
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Nucleolar protein nucleolin functions in replication stress-induced DNA damage responses.
Kawamura K, Qi F, Meng Q, Hayashi I, Kobayashi J.
Journal of Radiation Research 2019 May; 60(3):281.
Application:IF, WB-Tr, Human, U-2 OS cells.
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Maternal gene Ooep may participate in homologous recombination-mediated DNA double-strand break repair in mouse oocytes.
He DJ, Wang L, Zhang ZB, Guo K, Li JZ, He XC, Cui QH, Zheng P.
Zoological Research 2018 Nov; 39(6):387.
Application:IF, WB, Mouse, Mouse Oocyte.
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A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability.
Tan SLW, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, Constantinou S, Renaudin X, Lee M, Aebersold R, Venkitaraman AR.
Cell 2017 Jun; 169(6):1105.
Application:IF, Human, HeLa cells.
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RecA bundles mediate homology pairing between distant sisters during DNA break repair.
Lesterlin C, Ball G, Schermelleh L, Sherratt DJ.
Nature 2014 Feb; 506(7487):249.
Application:ICC, Bacteria, E. coli K12 TB28.
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A cancer-associated BRCA2 mutation reveals masked nuclear export signals controlling localization.
Jeyasekharan AD, Liu Y, Hattori H, Pisupati V, Jonsdottir AB, Rajendra E, Lee M, Sundaramoorthy E, Schlachter S, Kaminski CF, Rosenfeld Y, Sato K, Savill J, Ayoub N, Venkitaraman AR.
Nature Structural & Molecular Biology 2013 Oct; 20(10):1191.
Application:IF, Human, 293T cells.
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DNA binding and RAD51 engagement by the BRCA2 C-terminus orchestrate DNA repair and replication fork preservation.
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