RAD23B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAD23B full-length
Host
Human
Theoretical MW (kDa)
45.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAD23B antibody (H00005887-B02) by Western Blots.
SDS-PAGE Gel
RAD23B transfected lysate.
Western Blot
Lane 1: RAD23B transfected lysate ( 45.1 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAD23B
Entrez GeneID
5887GeneBank Accession#
BC020973Protein Accession#
AAH20973Gene Name
RAD23B
Gene Alias
HHR23B, HR23B, P58
Gene Description
RAD23 homolog B (S. cerevisiae)
Omim ID
600062Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
Other Designations
OTTHUMP00000021857|RAD23, yeast homolog of, B|UV excision repair protein RAD23 homolog B|XP-C repair complementing complex 58 kDa|XP-C repair complementing protein
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Interactome
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