QARS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-QARS full-length
Host
Human
Theoretical MW (kDa)
85.36
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-QARS antibody (H00005859-B01) by Western Blots.
SDS-PAGE Gel
QARS transfected lysate.
Western Blot
Lane 1: QARS transfected lysate ( 85.36 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — QARS
Entrez GeneID
5859GeneBank Accession#
NM_005051.1Protein Accession#
NP_005042.1Gene Name
QARS
Gene Alias
GLNRS, PRO2195
Gene Description
glutaminyl-tRNA synthetase
Omim ID
603727Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. In metazoans, 9 aminoacyl-tRNA synthetases specific for glutamine (gln), glutamic acid (glu), and 7 other amino acids are associated within a multienzyme complex. Although present in eukaryotes, glutaminyl-tRNA synthetase (QARS) is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln). Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq
Other Designations
glutamine tRNA ligase|glutamine-tRNA synthetase
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Interactome
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Pathway
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