PYGB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PYGB transfected lysate.
Western Blot
Lane 1: PYGB transfected lysate ( 96.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PYGB full-length
Host
Human
Theoretical MW (kDa)
96.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PYGBantibody (H00005834-D01)byWesternBlots.
SDS-PAGE Gel
PYGB transfected lysate.
Western Blot
Lane 1: PYGB transfected lysate ( 96.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PYGB
Entrez GeneID
5834GeneBank Accession#
NM_002862Protein Accession#
NP_002853.2Gene Name
PYGB
Gene Alias
MGC9213
Gene Description
phosphorylase, glycogen; brain
Omim ID
138550Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq
Other Designations
OTTHUMP00000030488|brain glycogen phosphorylase|glycogen phosphorylase B
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Interactome
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Pathway
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Disease
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