PYCR1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PYCR1 transfected lysate.
Western Blot
Lane 1: PYCR1 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PYCR1 full-length
Host
Human
Theoretical MW (kDa)
35.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PYCR1 antibody (H00005831-B01) by Western Blots.
SDS-PAGE Gel
PYCR1 transfected lysate.
Western Blot
Lane 1: PYCR1 transfected lysate ( 35.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PYCR1
Entrez GeneID
5831GeneBank Accession#
NM_006907.2Protein Accession#
NP_008838.2Gene Name
PYCR1
Gene Alias
P5C, P5CR, PIG45, PP222, PYCR
Gene Description
pyrroline-5-carboxylate reductase 1
Omim ID
179035Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
P5C reductase|proliferation-inducing protein 45
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Interactome
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