PTPN7 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PTPN7 transfected lysate.
Western Blot
Lane 1: PTPN7 transfected lysate ( 40.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PTPN7 full-length
Host
Human
Theoretical MW (kDa)
40.5
Interspecies Antigen Sequence
Mouse (92); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PTPN7 antibody (H00005778-D01P) by Western Blots.
SDS-PAGE Gel
PTPN7 transfected lysate.
Western Blot
Lane 1: PTPN7 transfected lysate ( 40.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PTPN7
Entrez GeneID
5778GeneBank Accession#
NM_002832.2Protein Accession#
NP_002823.2Gene Name
PTPN7
Gene Alias
BPTP-4, HEPTP, LC-PTP, LPTP, PTPNI
Gene Description
protein tyrosine phosphatase, non-receptor type 7
Omim ID
176889Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and suppress the MAP kinase activities. This PTP was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000034115|dual specificity phosphatase 1|hematopoietic protein-tyrosine phosphatase|protein-tyrosine phoshatase, nonreceptor-type, stress induced
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