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Last updated: 2017/6/25
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PTK9 monoclonal antibody (M05), clone 1D12

  • Catalog # : H00005756-M05
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant PTK9.
  • Immunogen:
  • PTK9 (AAH22344, 80 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • VAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSSAKPKGPAGKRGIRRLIRGPAETEATTD
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00005756-M05
    Western Blot detection against Immunogen (44.66 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of PTK9 expression in transfected 293T cell line by PTK9 monoclonal antibody (M05), clone 1D12.

    Lane 1: PTK9 transfected lysate(43.9 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged PTK9 is approximately 0.03ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of PTK9 over-expressed 293 cell line, cotransfected with PTK9 Validated Chimera RNAi ( Cat # H00005756-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTK9 monoclonal antibody (M05) clone 1D12 (Cat # H00005756-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 5756
  • Gene Name:
  • TWF1
  • Gene Alias:
  • A6,MGC23788,MGC41876,PTK9
  • Gene Description:
  • twinfilin, actin-binding protein, homolog 1 (Drosophila)
  • Gene Summary:
  • This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq
  • Other Designations:
  • A6 protein tyrosine kinase,PTK9 protein tyrosine kinase 9,protein tyrosine kinase 9,twinfilin 1
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