PTGIS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PTGIS transfected lysate.
Western Blot
Lane 1: PTGIS transfected lysate ( 55.11 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PTGIS full-length
Host
Human
Theoretical MW (kDa)
55.11
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PTGIS antibody (H00005740-B01) by Western Blots.
SDS-PAGE Gel
PTGIS transfected lysate.
Western Blot
Lane 1: PTGIS transfected lysate ( 55.11 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PTGIS
Entrez GeneID
5740GeneBank Accession#
NM_000961.3Protein Accession#
NP_000952.1Gene Name
PTGIS
Gene Alias
CYP8, CYP8A1, MGC126858, MGC126860, PGIS, PTGI
Gene Description
prostaglandin I2 (prostacyclin) synthase
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. However, this protein is considered a member of the cytochrome P450 superfamily on the basis of sequence similarity rather than functional similarity. This endoplasmic reticulum membrane protein catalyzes the conversion of prostglandin H2 to prostacyclin (prostaglandin I2), a potent vasodilator and inhibitor of platelet aggregation. An imbalance of prostacyclin and its physiological antagonist thromboxane A2 contribute to the development of myocardial infarction, stroke, and atherosclerosis. [provided by RefSeq
Other Designations
OTTHUMP00000031777|cytochrome P450, family 8, subfamily A, polypeptide 1
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