PSMD7 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSMD7 full-length
Host
Human
Theoretical MW (kDa)
37
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSMD7 antibody (H00005713-B01) by Western Blots.
SDS-PAGE Gel
PSMD7 transfected lysate.
Western Blot
Lane 1: PSMD7 transfected lysate ( 37 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PSMD7
Entrez GeneID
5713GeneBank Accession#
NM_002811Protein Accession#
NP_002802Gene Name
PSMD7
Gene Alias
MOV34, P40, Rpn8, S12
Gene Description
proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
Omim ID
157970Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. [provided by RefSeq
Other Designations
26S proteasome non-ATPase regulatory subunit 7|26S proteasome regulatory subunit S12|Moloney leukemia virus-34 proviral integration|Mov34 homolog|proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog)|proteasome 26S non-ATPase subunit
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Interactome
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Disease
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