PSMD5 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PSMD5 transfected lysate.
Western Blot
Lane 1: PSMD5 transfected lysate ( 55.55 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSMD5 full-length
Host
Human
Theoretical MW (kDa)
55.55
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSMD5 antibody (H00005711-B01) by Western Blots.
SDS-PAGE Gel
PSMD5 transfected lysate.
Western Blot
Lane 1: PSMD5 transfected lysate ( 55.55 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PSMD5
Entrez GeneID
5711GeneBank Accession#
NM_005047.2Protein Accession#
NP_005038.1Gene Name
PSMD5
Gene Alias
KIAA0072, MGC23145, S5B
Gene Description
proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Omim ID
604452Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base. [provided by RefSeq
Other Designations
26S protease subunit S5 basic|26S proteasome non-ATPase regulatory subunit 5|26S proteasome subunit S5B|OTTHUMP00000021990|proteasome 26S non-ATPase subunit 5
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