PSMD5 monoclonal antibody (M17), clone 1H7
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PSMD5 expression in transfected 293T cell line by PSMD5 monoclonal antibody (M17), clone 1H7.
Lane 1: PSMD5 transfected lysate (Predicted MW: 56.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot detection against Immunogen (80.96 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PSMD5.
Immunogen
PSMD5 (AAH14478.1, 1 a.a. ~ 504 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAQALALLREVARLEAPLEELRALHSVLQAVPLNELRQQAAELRLGPLFSLLNENHREKTTLCVSILERLLQAMEPVHVARNLRVDLQRGLIHPDDSVKILTLSQIGRIVENSDAVTEILNNAELLKQIVYCIGGENLSVAKAAIKSLSRISLTQAGLEALFESNLLDDLKSVMKTNDIVRYRVYELIIEISSVSPESLNYCTTSGLVTQLLRELTGEDVLVRATCIEMVTSLAYTHHGRQYLAQEGVIDQISNIIVGADSDPFSSFYLPGFVKFFGNLAVMDSPQQICERYPIFVEKVFEMIESQDPTMIGVAVDTVGILGSNVEGKQVLQKTGTRFERLLMRIGHQSKNAPVELKIRCLDAISSLLYLPPEQQTDDLLRMTESWFSSLSRDPLELFRGISSQPFPELHCAALKVFTAIANQPWAQKLMFNSPGFVEYVVDRSVEHDKASKDAKYELVKALANSKTIAEIFGNPNYLRLRTYLSEGPYYVKPVSTTAVEGAE
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (80.96 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PSMD5 expression in transfected 293T cell line by PSMD5 monoclonal antibody (M17), clone 1H7.
Lane 1: PSMD5 transfected lysate (Predicted MW: 56.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
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Gene Info — PSMD5
Entrez GeneID
5711GeneBank Accession#
BC014478Protein Accession#
AAH14478.1Gene Name
PSMD5
Gene Alias
KIAA0072, MGC23145, S5B
Gene Description
proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
Omim ID
604452Gene Ontology
HyperlinkGene Summary
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator base. [provided by RefSeq
Other Designations
26S protease subunit S5 basic|26S proteasome non-ATPase regulatory subunit 5|26S proteasome subunit S5B|OTTHUMP00000021990|proteasome 26S non-ATPase subunit 5
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