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PSMC4 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005704-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PSMC4 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 47.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PSMC4 antibody (H00005704-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005704-T01
    PSMC4 transfected lysate.
    Western Blot
    QC Testing of H00005704-T01
    Lane 1: PSMC4 transfected lysate ( 47.4 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5704
  • Gene Name:
  • PSMC4
  • Gene Alias:
  • MGC13687,MGC23214,MGC8570,MIP224,S6,TBP7
  • Gene Description:
  • proteasome (prosome, macropain) 26S subunit, ATPase, 4
  • Gene Summary:
  • The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit has been shown to interact with an orphan member of the nuclear hormone receptor superfamily highly expressed in liver, and with gankyrin, a liver oncoprotein. Two transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • MB67 interacting protein,Tat-binding protein 7,protease 26S subunit 6,proteasome 26S ATPase subunit 4
  • Gene Pathway
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