PSMB3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PSMB3 transfected lysate.
Western Blot
Lane 1: PSMB3 transfected lysate ( 22.66 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PSMB3 full-length
Host
Human
Theoretical MW (kDa)
22.66
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PSMB3 antibody (H00005691-B01) by Western Blots.
SDS-PAGE Gel
PSMB3 transfected lysate.
Western Blot
Lane 1: PSMB3 transfected lysate ( 22.66 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PSMB3
Entrez GeneID
5691GeneBank Accession#
NM_002795.2Protein Accession#
NP_002786.2Gene Name
PSMB3
Gene Alias
HC10-II, MGC4147
Gene Description
proteasome (prosome, macropain) subunit, beta type, 3
Omim ID
602176Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12. [provided by RefSeq
Other Designations
proteasome beta 3 subunit|proteasome chain 13|proteasome component C10-II|proteasome theta chain
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