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Last updated: 2017/9/17

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PSAP 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005660-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PSAP full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 57.75
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PSAP antibody (H00005660-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005660-T01
    PSAP transfected lysate.
    Western Blot
    QC Testing of H00005660-T01
    Lane 1: PSAP transfected lysate ( 57.75 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5660
  • Gene Name:
  • PSAP
  • Gene Alias:
  • FLJ00245,GLBA,MGC110993,SAP1
  • Gene Description:
  • prosaposin
  • Gene Summary:
  • This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000019776,sphingolipid activator protein-1
  • Gene Pathway
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