PSAP monoclonal antibody (M03), clone 2F6
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PSAP.
Immunogen
PSAP (AAH04275, 1 a.a. ~ 524 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDITEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPLAHKPLLGTEKCIWGPSYWCQNTETAAQCNAVEHCKRHVWN
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (83.16 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
-
Applications
Western Blot (Transfected lysate)
Western Blot analysis of PSAP expression in transfected 293T cell line by PSAP monoclonal antibody (M03), clone 2F6.
Lane 1: PSAP transfected lysate (Predicted MW: 58.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PSAP is 0.1 ng/ml as a capture antibody.ELISA
-
Gene Info — PSAP
Entrez GeneID
5660GeneBank Accession#
BC004275Protein Accession#
AAH04275Gene Name
PSAP
Gene Alias
FLJ00245, GLBA, MGC110993, SAP1
Gene Description
prosaposin
Gene Ontology
HyperlinkGene Summary
This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000019776|sphingolipid activator protein-1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning.
Mai Ly Tran, Johanna Tüshaus , Yeongho Kim, Bulat R Ramazanov, Swathi Devireddy, Stefan F Lichtenthaler, Shawn M Ferguson, Julia von Blume.
Traffic (Copenhagen, Denmark) 2023 Jan; 24(1):4.
Application:WB-Tr, Human, HeLa cells.
-
Co-localization of cystatin C and prosaposin in cultured neurons and in anterior horn neurons with amyotrophic lateral sclerosis.
Wada Y, Nagai A, Sheikh AM, Onoda K, Terashima M, Shiota Y, Araki A, Yamaguchi S.
Journal of the Neurological Sciences 2018 Jan; 384:67.
Application:IF, WB-Re, Human, A1 neuronal hybrid cell line, Recombinant protein, Spinal cord from patients with amyotrophic lateral sclerosis.
-
Cab45 deficiency leads to the mistargeting of progranulin and prosaposin and aberrant lysosomal positioning.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com