EIF2AK2 monoclonal antibody (M01), clone 1B9
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant EIF2AK2.
Immunogen
EIF2AK2 (NP_002750, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGN
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
EIF2AK2 monoclonal antibody (M01), clone 1B9. Western Blot analysis of EIF2AK2 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Transfected lysate)
Western Blot analysis of EIF2AK2 expression in transfected 293T cell line by EIF2AK2 monoclonal antibody (M01), clone 1B9.
Lane 1: EIF2AK2 transfected lysate(62.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — EIF2AK2
Entrez GeneID
5610GeneBank Accession#
NM_002759Protein Accession#
NP_002750Gene Name
EIF2AK2
Gene Alias
EIF2AK1, MGC126524, PKR, PRKR
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 2
Omim ID
176871Gene Ontology
HyperlinkGene Summary
interferon-inducible double stranded RNA dependent
Other Designations
OTTHUMP00000126954|double stranded RNA activated protein kinase|interferon-inducible elF2alpha kinase|protein kinase, interferon-inducible double stranded RNA dependent
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Interactome
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Disease
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Publication Reference
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KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182.
Sharma NR, Majerciak V, Kruhlak MJ, Yu L, Kang JG, Yang A, Gu S, Fritzler MJ, Zheng ZM.
Nucleic Acids Research 2019 Sep; 47(17):9368.
Application:WB-Tr, Human, iSLK/Bac16 cells.
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KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182.
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