MAP2K6 monoclonal antibody (M02), clone 2F2
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More Files
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Western Blot (Cell lysate)
MAP2K6 monoclonal antibody (M02), clone 2F2 Western Blot analysis of MAP2K6 expression in COLO 320 HSR ( Cat # L020V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAP2K6 is approximately 0.3ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAP2K3 and MAP2K6. HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP2K6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAP2K6 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAP2K6.
Immunogen
MAP2K6 (AAH12009, 231 a.a. ~ 334 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
QKGYSVKSDIWSLGITMIELAILRFPYDSWGTPFQQLKQVVEEPSPQLPADKFSAEFVDFTSQCLKKNSKERPTYPELMQHPFFTLHESKGTDVASFVKLILGD
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAP2K6 monoclonal antibody (M02), clone 2F2 Western Blot analysis of MAP2K6 expression in COLO 320 HSR ( Cat # L020V1 ).Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAP2K6 is approximately 0.3ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between MAP2K3 and MAP2K6. HeLa cells were stained with anti-MAP2K3 rabbit purified polyclonal 1:1200 and anti-MAP2K6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to MAP2K6 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MAP2K6
Entrez GeneID
5608GeneBank Accession#
BC012009Protein Accession#
AAH12009Gene Name
MAP2K6
Gene Alias
MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3
Gene Description
mitogen-activated protein kinase kinase 6
Omim ID
601254Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the dual specificity protein kinase family, which functions as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAP kinase mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6)
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Interactome
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Pathway
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Disease
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