MAP2K5 (Human) IP-WB Antibody Pair
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Immunoprecipitation of MAP2K5 transfected lysate using rabbit polyclonal anti-MAP2K5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-MAP2K5.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97%); Rat (98%)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of MAP2K5 transfected lysate using rabbit polyclonal anti-MAP2K5 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-MAP2K5.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-MAP2K5 (300 ul)
2. Antibody pair for WB: mouse polyclonal anti-MAP2K5 (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — MAP2K5
Entrez GeneID
5607Gene Name
MAP2K5
Gene Alias
HsT17454, MAPKK5, MEK5, PRKMK5
Gene Description
mitogen-activated protein kinase kinase 5
Omim ID
602520Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase specifically interacts with and activates MAPK7/ERK5. This kinase itself can be phosphorylated and activated by MAP3K3/MEKK3, as well as by atypical protein kinase C isoforms (aPKCs). The signal cascade mediated by this kinase is involved in growth factor stimulated cell proliferation and muscle cell differentiation. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
MAP kinase kinase MEK5b|MAPK/ERK kinase 5|dual specificity mitogen-activated protein kinase kinase 5
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Interactome
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Disease
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