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MAP2K3 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005606-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MAP2K3 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 38.28
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MAP2K3 antibody (H00005606-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005606-T01
    MAP2K3 transfected lysate.
    Western Blot
    QC Testing of H00005606-T01
    Lane 1: MAP2K3 transfected lysate ( 38.28 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5606
  • Gene Name:
  • MAP2K3
  • Gene Alias:
  • Gene Description:
  • mitogen-activated protein kinase kinase 3
  • Gene Summary:
  • The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq
  • Other Designations:
  • MAP kinase kinase 3,MAPK/ERK kinase 3,OTTHUMP00000166044,dual specificity mitogen activated protein kinase kinase 3
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