MAPK9 monoclonal antibody (M02), clone 2F8
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More Files
- More Functions
Western Blot (Cell lysate)
MAPK9 monoclonal antibody (M02), clone 2F8 Western Blot analysis of MAPK9 expression in HeLa ( Cat # L013V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK9 is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TRAF2 and MAPK9. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.07 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAPK9.
Immunogen
MAPK9 (AAH32539, 321 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR
Host
Mouse
Reactivity
Human
Isotype
IgG1 Lambda
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.07 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
MAPK9 monoclonal antibody (M02), clone 2F8 Western Blot analysis of MAPK9 expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK9 is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between TRAF2 and MAPK9. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-MAPK9 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MAPK9
Entrez GeneID
5601GeneBank Accession#
BC032539Protein Accession#
AAH32539Gene Name
MAPK9
Gene Alias
JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK
Gene Description
mitogen-activated protein kinase 9
Omim ID
602896Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
Jun kinase|MAP kinase 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|mitogen-activated protein kinase 9 isoform JNK2 alpha2|stress-activated protein kinase JNK2
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Interactome
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Pathway
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Disease
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