MAPK9 monoclonal antibody (M01), clone 1C1-3A8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody (M01), clone 1C1-3A8.
Lane 1: MAPK9 transfected lysate(48.1 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK9 is approximately 3ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi ( Cat # H00005601-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody (M01) clone 1C1-3A8 (Cat # H00005601-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (72.27 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MAPK9.
Immunogen
MAPK9 (AAH32539, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMSTEQTLASDTDSSLDASTGPLEGCR
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (72.27 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAPK9 expression in transfected 293T cell line by MAPK9 monoclonal antibody (M01), clone 1C1-3A8.
Lane 1: MAPK9 transfected lysate(48.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MAPK9 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAPK9 is approximately 3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MAPK9 over-expressed 293 cell line, cotransfected with MAPK9 Validated Chimera RNAi ( Cat # H00005601-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK9 monoclonal antibody (M01) clone 1C1-3A8 (Cat # H00005601-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MAPK9
Entrez GeneID
5601GeneBank Accession#
BC032539Protein Accession#
AAH32539Gene Name
MAPK9
Gene Alias
JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK
Gene Description
mitogen-activated protein kinase 9
Omim ID
602896Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
Jun kinase|MAP kinase 9|c-Jun N-terminal kinase 2|c-Jun kinase 2|mitogen-activated protein kinase 9 isoform JNK2 alpha2|stress-activated protein kinase JNK2
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