PRKDC monoclonal antibody (M02), clone 1B9
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PRKDC.
Immunogen
PRKDC (NP_008835, 4019 a.a. ~ 4128 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGREPWM
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PRKDC monoclonal antibody (M02), clone 1B9 Western Blot analysis of PRKDC expression in A-431 ( Cat # L015V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PRKDC
Entrez GeneID
5591GeneBank Accession#
NM_006904Protein Accession#
NP_008835Gene Name
PRKDC
Gene Alias
DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350
Gene Description
protein kinase, DNA-activated, catalytic polypeptide
Omim ID
600899Gene Ontology
HyperlinkGene Summary
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM
Other Designations
hyper-radiosensitivity of murine scid mutation, complementing 1
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Interactome
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Pathway
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Disease
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Publication Reference
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A phase Ib study of the DNA-PK inhibitor peposertib combined with neoadjuvant chemoradiation in patients with locally advanced rectal cancer.
Paul B Romesser, Jaume Capdevila, Rocio Garcia-Carbonero, Tony Philip, Carlos Fernández-Martos, Richard Tuli, Almudena Rodriguez-Gutierrez, Mirjam Kuipers, Andreas Becker, Anna Coenen-Stass, Barbara Sarholz, Xiaoli You, Eric D Miller.
Clinical Cancer Research 2023 Dec; [Epub]:0.
Application:WB, Human, PBMC cells.
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Pharmacologic Inhibitor of DNA-PK, M3814, Potentiates Radiotherapy and Regresses Human Tumors in Mouse Models.
Frank T Zenke, Astrid Zimmermann, Christian Sirrenberg, Heike Dahmen, Vladimir Kirkin, Ulrich Pehl, Thomas Grombacher, Claudia Wilm, Thomas Fuchss, Christiane Amendt, Lyubomir T Vassilev, Andree Blaukat.
Molecular Cancer Therapeutics 2020 Mar; 19(5):1091.
Application:Quant, Mouse, tumor.
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Quantification of the Host Response Proteome after Herpes Simplex 1 Virus infection.
Berard AR, Coombs KM, Severini A.
Journal of Proteome Research 2015 May; 14(5):2121.
Application:WB, Human, HEK293 cells.
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A phase Ib study of the DNA-PK inhibitor peposertib combined with neoadjuvant chemoradiation in patients with locally advanced rectal cancer.
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