This antibody pair set comes with matched antibody pair to detect and quantify protein level of human PRKCZ.
Quality Control Testing:
Standard curve using PRKCZ 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the PRKCZ 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-PRKCZ (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-PRKCZ (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq