PKN1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PKN1 transfected lysate.
Western Blot
Lane 1: PKN1 transfected lysate ( 103.73 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PKN1 full-length
Host
Human
Theoretical MW (kDa)
103.73
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PKN1 antibody (H00005585-B01) by Western Blots.
SDS-PAGE Gel
PKN1 transfected lysate.
Western Blot
Lane 1: PKN1 transfected lysate ( 103.73 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PKN1
Entrez GeneID
5585GeneBank Accession#
BC040061.1Protein Accession#
AAH40061.1Gene Name
PKN1
Gene Alias
DBK, MGC46204, PAK1, PKN, PKN-ALPHA, PRK1, PRKCL1
Gene Description
protein kinase N1
Omim ID
601032Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
protein kinase C-like 1|protein kinase C-like PKN|protein kinase C-related kinase 1|serine-threonine kinase N|serine/threonine protein kinase N
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Interactome
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Disease
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