PRKCD monoclonal antibody (M02), clone 6A2
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.
Lane 1: PRKCD transfected lysate(77.5 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of PRKCD over-expressed 293 cell line, cotransfected with PRKCD Validated Chimera RNAi ( Cat # H00005580-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCD monoclonal antibody (M02) clone 6A2 (Cat # H00005580-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PRKCD.
Immunogen
PRKCD (NP_006245, 577 a.a. ~ 676 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (81)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PRKCD expression in transfected 293T cell line by PRKCD monoclonal antibody (M02), clone 6A2.
Lane 1: PRKCD transfected lysate(77.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRKCD is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PRKCD over-expressed 293 cell line, cotransfected with PRKCD Validated Chimera RNAi ( Cat # H00005580-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCD monoclonal antibody (M02) clone 6A2 (Cat # H00005580-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — PRKCD
Entrez GeneID
5580GeneBank Accession#
NM_006254Protein Accession#
NP_006245Gene Name
PRKCD
Gene Alias
MAY1, MGC49908, PKCD, nPKC-delta
Gene Description
protein kinase C, delta
Omim ID
176977Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
protein kinase C delta VIII
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Interactome
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Pathway
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Disease
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