PRKCB1 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRKCB1 full-length
Host
Human
Theoretical MW (kDa)
76.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRKCB1 antibody (H00005579-D01P) by Western Blots.
SDS-PAGE Gel
PRKCB transfected lysate.
Western Blot
Lane 1: PRKCB transfected lysate ( 76.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRKCB
Entrez GeneID
5579GeneBank Accession#
NM_212535.1Protein Accession#
NP_997700.1Gene Name
PRKCB
Gene Alias
MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2
Gene Description
protein kinase C, beta
Omim ID
176970Gene Ontology
HyperlinkGene Summary
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
protein kinase C, beta 1 polypeptide
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Interactome
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Pathway
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Disease
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