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Last updated: 2016/12/4
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PRKAR2A 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005576-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRKAR2A full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 42.13
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRKAR2A antibody (H00005576-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005576-T01
    PRKAR2A transfected lysate.
    Western Blot
    QC Testing of H00005576-T01
    Lane 1: PRKAR2A transfected lysate ( 42.13 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5576
  • Gene Name:
  • PRKAR2A
  • Gene Alias:
  • MGC3606,PKR2,PRKAR2
  • Gene Description:
  • protein kinase, cAMP-dependent, regulatory, type II, alpha
  • Gene Summary:
  • cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). [provided by RefSeq
  • Other Designations:
  • cAMP-dependent protein kinase regulatory subunit RII alpha,cAMP-dependent protein kinase, regulatory subunit alpha 2,protein kinase A, RII-alpha subunit
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