PRKAR1B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: PRKAR1B transfected lysate ( 43.1 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
PRKAR1B transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRKAR1B full-length
Host
Human
Theoretical MW (kDa)
43.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRKAR1B antibody (H00005575-B01) by Western Blots.
Western Blot
Lane 1: PRKAR1B transfected lysate ( 43.1 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PRKAR1B transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRKAR1B
Entrez GeneID
5575GeneBank Accession#
NM_002735Protein Accession#
NP_002726Gene Name
PRKAR1B
Gene Alias
PRKAR1
Gene Description
protein kinase, cAMP-dependent, regulatory, type I, beta
Omim ID
176911Gene Ontology
HyperlinkGene Summary
Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM
Other Designations
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Interactome
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Pathway
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