PRKAR1A 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRKAR1A full-length
Host
Human
Theoretical MW (kDa)
42.02
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRKAR1A antibody (H00005573-B01) by Western Blots.
Western Blot
Lane 1: PRKAR1A transfected lysate ( 43 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
PRKAR1A transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRKAR1A
Entrez GeneID
5573GeneBank Accession#
NM_002734Protein Accession#
NP_002725Gene Name
PRKAR1A
Gene Alias
CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, TSE1
Gene Description
protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
Gene Ontology
HyperlinkGene Summary
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
cAMP-dependent protein kinase regulatory subunit RIalpha|cAMP-dependent protein kinase type I-alpha regulatory chain|cAMP-dependent protein kinase, regulatory subunit alpha 1|protein kinase A type 1a regulatory subunit|tissue-specific extinguisher 1
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