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Last updated: 2017/8/13
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PRKACA 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005566-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRKACA full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 40.6
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRKACA antibody (H00005566-D01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005566-T02
    PRKACA transfected lysate.
    Western Blot
    QC Testing of H00005566-T02
    Lane 1: PRKACA transfected lysate ( 40.60 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5566
  • Gene Name:
  • PRKACA
  • Gene Alias:
  • MGC102831,MGC48865,PKACA
  • Gene Description:
  • protein kinase, cAMP-dependent, catalytic, alpha
  • Gene Summary:
  • cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • PKA C-alpha,cAMP-dependent protein kinase catalytic subunit alpha,cAMP-dependent protein kinase catalytic subunit alpha, isoform 1,protein kinase A catalytic subunit
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