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Last updated: 2017/3/19
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PRKAB1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005564-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRKAB1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 30.4
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRKAB1 antibody (H00005564-D01P) by Western Blots.
    Western Blot
    QC Testing of H00005564-T01
    Lane 1: PRKAB1 transfected lysate ( 30.40 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00005564-T01
    PRKAB1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5564
  • Gene Name:
  • PRKAB1
  • Gene Alias:
  • AMPK,HAMPKb,MGC17785
  • Gene Description:
  • protein kinase, AMP-activated, beta 1 non-catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase beta-1 subunit,AMP-activated protein kinase beta 1 non-catalytic subunit,AMP-activated protein kinase beta subunit,AMPK beta -1 chain,AMPK beta 1,protein kinase, AMP-activated, noncatalytic, beta-1
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