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Last updated: 2016/12/4
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PRKAA2 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005563-T02
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRKAA2 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 62.3
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRKAA2 antibody (H00005563-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00005563-T02
    PRKAA2 transfected lysate.
    Western Blot
    QC Testing of H00005563-T02
    Lane 1: PRKAA2 transfected lysate ( 62.30 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5563
  • Gene Name:
  • PRKAA2
  • Gene Alias:
  • AMPK,AMPK2,PRKAA
  • Gene Description:
  • protein kinase, AMP-activated, alpha 2 catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, catalytic alpha-2 chain,AMP-activated protein kinase alpha 2 catalytic subunit,AMPK-alpha-2 chain,OTTHUMP00000009993
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