PRKAA2 (Human) Recombinant Protein (P01)
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More Files
- More Functions
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Specification
Product Description
Human PRKAA2 full-length ORF ( NP_006243.2, 1 a.a. - 552 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
88.7
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PRKAA2
Entrez GeneID
5563GeneBank Accession#
NM_006252.2Protein Accession#
NP_006243.2Gene Name
PRKAA2
Gene Alias
AMPK, AMPK2, PRKAA
Gene Description
protein kinase, AMP-activated, alpha 2 catalytic subunit
Omim ID
600497Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase, catalytic alpha-2 chain|AMP-activated protein kinase alpha 2 catalytic subunit|AMPK-alpha-2 chain|OTTHUMP00000009993
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Interactome
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Pathway
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Disease
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Publication Reference
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Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.
Peng Jiang, Lejiao Ren, Li Zhi, Zhong Yu, Fengxiang Lv, Fengli Xu, Wei Peng, Xiaoyu Bai, Kunlun Cheng, Li Quan, Xiuqin Zhang, Xianhua Wang, Yan Zhang, Dan Yang, Xinli Hu, Rui-Ping Xiao.
Molecular Cell 2021 Jan; 81(3):629.
Application:PI, WB-Re, Recombinant protein.
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Adipocyte-specific deletion of Ip6k1 reduces diet-induced obesity by enhancing AMPK-mediated thermogenesis.
Zhu Q, Ghoshal S, Rodrigues A, Gao S, Asterian A, Kamenecka TM, Barrow JC, Chakraborty A.
The Journal of Clinical Investigation 2016 Oct; 126(11):4273.
Application:WB, Human, Recombinant protein.
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Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.
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