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Last updated: 2017/3/19
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PRKAA1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005562-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRKAA1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 65.5
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PTPRFantibody (H00005562-B01P)byWesternBlots.
    SDS-PAGE Gel
    QC Testing of H00005562-T02
    PRKAA1 transfected lysate.
    Western Blot
    QC Testing of H00005562-T02
    Lane 1: PRKAA1 transfected lysate ( 65.5 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5562
  • Gene Name:
  • PRKAA1
  • Gene Alias:
  • AMPK,AMPKa1,MGC33776,MGC57364
  • Gene Description:
  • protein kinase, AMP-activated, alpha 1 catalytic subunit
  • Gene Summary:
  • The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
  • Other Designations:
  • 5'-AMP-activated protein kinase, catalytic alpha-1 chain,AMP -activate kinase alpha 1 subunit,AMP-activated protein kinase, catalytic, alpha-1,AMPK alpha 1
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