PRF1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human PRF1 full-length ORF ( AAH63043.1, 22 a.a. - 555 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
PCHTAARSECKRSHKFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPDGTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRHVTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPTSNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVECRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQPAYLRLISNYGTHFIRAVELGGRISALTALRTCELALEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACEEKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLLFGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLLDSQDPRREALRRALSQYLTDRARWRDCSRPCPPGRQKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVTFIQAWGLWGDWFTATDAYVKLFLGGQELRTSTVWDNNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRDDDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARCLPHLGGGTCLDYVPQMLLGEPPGNRSGAVW
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
84.48
Interspecies Antigen Sequence
Mouse (68); Rat (69)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — PRF1
Entrez GeneID
5551GeneBank Accession#
BC063043Protein Accession#
AAH63043.1Gene Name
PRF1
Gene Alias
FLH2, HPLH2, MGC65093, P1, PFN1, PFP
Gene Description
perforin 1 (pore forming protein)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq
Other Designations
OTTHUMP00000019759|cytolysin|lymphocyte pore forming protein|perforin 1
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Interactome
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Pathway
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Disease
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Publication Reference
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CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection.
Jian Cheng, Zhao Liu, Tian Deng, Zhen Lu, Maoxuan Liu, Xiaoxu Lu, Funmilayo Oladunni Adeshakin, Dehong Yan, Guizhong Zhang, Xiaochun Wan.
Molecular Immunology 2021 Jan; 129:94.
Application:Func, Human, HeLa, HepG2 cells.
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Role of the inflammatory protein serine protease inhibitor Kazal in preventing cytolytic granule granzyme A-mediated apoptosis.
Lu F, Lamontagne J, Sun A, Pinkerton M, Block T, Lu X.
Immunology 2011 Dec; 134(4):398.
Application:Incubated, Recombinant protein.
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CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection.
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