PREP monoclonal antibody (M04), clone 2D7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PREP.
Immunogen
PREP (NP_002717.3, 611 a.a. ~ 710 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LVKYSPLHNVKLPEADDIQYPSMLLLTADHDDRVVPLHSLKFIATLQYIVGRSRKQSNPLLIHVDTKAGHGAGKPTAKVIEEVSDMFAFIARCLNVDWIP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PREP is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PREP on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PREP
Entrez GeneID
5550GeneBank Accession#
NM_002726Protein Accession#
NP_002717.3Gene Name
PREP
Gene Alias
MGC16060, PE, PEP
Gene Description
prolyl endopeptidase
Omim ID
600400Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq
Other Designations
OTTHUMP00000016916|OTTHUMP00000040498|dJ355L5.1 (prolyl endopeptidase)|post-proline cleaving enzyme|prolyl oligopeptidase
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Interactome
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Disease
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Publication Reference
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Renal release of Ac-SDKP is part of an antifibrotic peptidergic system in the kidney.
Romero CA, Kumar N, Nakagawa P, Worou ME, Liao TD, Peterson EL, Carretero OA.
American Journal of Physiology. Renal Physiology 2018 Nov; [Epub].
Application:IS, Rat, Kidney.
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Renal release of Ac-SDKP is part of an antifibrotic peptidergic system in the kidney.
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