PRELP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRELP transfected lysate.
Western Blot
Lane 1: PRELP transfected lysate ( 43.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRELP full-length
Host
Human
Theoretical MW (kDa)
43.8
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRELP antibody (H00005549-B01P) by Western Blots.
SDS-PAGE Gel
PRELP transfected lysate.
Western Blot
Lane 1: PRELP transfected lysate ( 43.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRELP
Entrez GeneID
5549GeneBank Accession#
NM_002725Protein Accession#
NP_002716.1Gene Name
PRELP
Gene Alias
MGC45323, MST161, MSTP161, SLRR2A
Gene Description
proline/arginine-rich end leucine-rich repeat protein
Omim ID
601914Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
Other Designations
55 kDa leucine-rich repeat protein of articular cartilage|OTTHUMP00000034099|prolargin proteoglycan|proline arginine-rich end leucine-rich repeat protein
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Interactome
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Disease
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