PRCP 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRCP transfected lysate.
Western Blot
Lane 1: PRCP transfected lysate ( 55.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRCP full-length
Host
Human
Theoretical MW (kDa)
55.8
Interspecies Antigen Sequence
Mouse (78); Rat (79)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRCP antibody (H00005547-B01P) by Western Blots.
SDS-PAGE Gel
PRCP transfected lysate.
Western Blot
Lane 1: PRCP transfected lysate ( 55.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRCP
Entrez GeneID
5547GeneBank Accession#
NM_005040Protein Accession#
NP_005031.1Gene Name
PRCP
Gene Alias
HUMPCP, MGC2202, PCP
Gene Description
prolylcarboxypeptidase (angiotensinase C)
Omim ID
176785Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a lysosomal prolylcarboxypeptidase, which cleaves C-terminal amino acids linked to proline in peptides such as angiotension II, III and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension II, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that this gene may be related to essential hypertension. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
Other Designations
angiotensinase C|lysosomal Pro-x carboxypeptidase|lysosomal carboxypeptidase C|proline carboxypeptidase|prolylcarboxypeptidase|prolylcarboxypeptidase isoform 1 preproprotein
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Interactome
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Disease
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